Study on the pharmacokinetics of Trilipix in Beagle dogs

OBJECTIVE To establish a method to determine the fenofibric acid in Beagle dog's plasma by HPLC,and to study the pharmacokinetics of Trilipix in Beagle dogs.METHODS The plasma samples were pretreated by acetonitrile.Nabumetone was used as internal standard.A Hypersil BDS C18 column(200 mm×4.6 mm,5 μm)with UV detection at 286 nm was used.The mobile phase was composed of 0.2% phosphoric acid-acetonitrile(50:50).The flow rate was 1.0 mL·min-1.six dogs were orally administered with a single dose of 1 Trilipix capsule(135 mg).The main pharmacokinetic parameters were calculated by DAS2.0 software.RESULTS The range of linearity was 0.20-40.00 μg·mL-1(r=0.999).The average recovery rates were 86.22%-98.94%.And the average extraction recovery rates were 57.72%-58.63%.Both the intra-day and inter-day RSD were less than 7.0%.After oral administration,the plasma concentration-time curve of fenofibric acid was well fitted with a two-compartment open model.The average main pharmacokinetic parameters,t1/2α was 1.57 h,t1/2βwas 13.25 h,V1/Fwas 8.28 L,Cl/F was 1.12 L·h-1,AUC(0-t) was 127.34 μg·h·mL-1,respectively.CONCLUSION The method is simple,rapid and accurate to be applied in pharmacokinetic study of Trilipix.