Effect of recombinant antisense c-myc adenovirus inhibiting proliferation and expression of PCNA in HL-60 cells

OBJECTIVE: To study recombinant antisense c-myc adenovirus(Ad-AS-c-myc) how to cause the alterations of c-myc and proliferating cell nuclear antigen expression in HL-60 cells.METHODS: The cultured HL-60 cells treated with Ad-AS-c-myc or Ad-LacZ with protamine sulfate were analysed by X-gal staining,MTT,RT-PCR and immunocytochemical techniques in vitro.RESULTS: The HL-60 cells were transducted effectively by Ad-LacZ+protamine sulfate(79.8%).The levels of c-myc transcription and the version were strongly inhibited by Ad-AS-c-myc in the transfected HL-60 cells.Ad-AS-c-myc strongly inhibited the cell growth in HL-60 cells(51%).Ad-AS-c-myc also decreased the expression of PCNA in HL-60 cells.CONCLUSIONS:Ad-AS-c-myc can inhibit the growth of HL-60 cells in vitro.Ad-AS-c-myc relates to the activity of PCNA and c-myc gene closly and takes a key role of biologic effects in HL-60 cells.Ad-AS-c-myc is a potential clinical utility in gene therapy for acute promyelocytic leukemia.