Comparison of mesenchymal stem cells from adipose tissue and bone marrow

Objective To compare adipose stromal cells(ASC) and bone marrow mesenchymal stem cells(MSC) and make a foundation for cell therapy based on ASCs.Methods Human adipose tissue was digested with collagenase type Ⅰ solution and ASCs were derived by culture.At the same time,(mononuclear) cells were isolated from human bone marrow and MSCs were derived by culture.Cells number was compared before and after the culture.The expression of surface antigens in two cells was detected by flow cytometry and the effects of ASCs or MSCs on mixed lymphocyte response and PHA-induced lymphocyte transformation were investigated.Results Even the number of(nucleated) cells isolated from bone marrow was significantly higher than from adipose tissue(P(0.01)).No significant differences were observed for yield of adherent stromal cells(P(0.05)).Both cells(expressed) CD13,CD29,CD44 and CD105.Difference in expression were noted for CD49d and CD106.The same number of ASCs and MSCs showed comparatively negative immunomodulatory functions by inhibiting the mixed lymphocyte response and induction of(transformation)(P(0.05)).Conclusions ASCs were similar to MSCs in the features of CD markers and(immunosuppressive)(properties),and this may make a foundation for the study of cell therapy based on ASCs.