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PCR-RFLP at growth hormone gene in yak and Holstein

Guifang Chen, Li Qifa, Qianba Yangzong, Tu Deng, Xie Zhuang

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Abstract

By polymerase chain reaction restriction fragment length polymorphism (PCR RFLP), growth hormone gene in Holstein and yak of Tibet was tested.A polymorphic AluⅠsite in the fifth exon at position 2141 was verified, one could be designated allele A and the other allele B. In yak and Holstein, the allele frequencies of A were 0 905 and 0 935 respectively, and those of B were 0 095 and 0 065 respectively without significant difference(P0 05).

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What this paper is about

By polymerase chain reaction restriction fragment length polymorphism (PCR RFLP), growth hormone gene in Holstein and yak of Tibet was tested.A polymorphic AluⅠsite in the fifth exon at position 2141 was verified, one could be designated allele A and the other allele B. In yak and Holstein, the allele frequencies of A were 0 905 and 0 935 respectively, and those of B were 0 095 and 0 065 respectively without significant difference(P0 05).

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Available abstract

By polymerase chain reaction restriction fragment length polymorphism (PCR RFLP), growth hormone gene in Holstein and yak of Tibet was tested.A polymorphic AluⅠsite in the fifth exon at position 2141 was verified, one could be designated allele A and the other allele B. In yak and Holstein, the allele frequencies of A were 0 905 and 0 935 respectively, and those of B were 0 095 and 0 065 respectively without significant difference(P0 05).

Key concepts: Restriction fragment length polymorphism, Biology, Allele, Exon, Polymorphism (computer science), Growth hormone, Genetics, Polymerase chain reaction

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