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1995•Chinese Journal of Coll BiologyRequires access

EFFECT OF ARTIFICIAL BASEMENT MEMBRANE ON THE GROWTH OF NASOPHARYNGEAL CARCINOMA CELL LINE (CNE-2)

Jnu Guangzhou

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Abstract

Artificial basement membrane (ABM) with spongiform configuration was mainly made of type I collagen (rat tail) which was formed into hydrated collagen lattices (HCLS), fibronectin, type IV collagen and laminin. ABM could cut off 10% of the fetal bovine serum (FBS) in medium, raise the viability of cells and prolong the period of subculture. The growth effecien-cy, plating effeciency and the rate of clonal growth of CNE-2 cells propagated on ABM with 2-5% FBS cultural medium were higher but the uptake of 3H-TdR was lower than that of these cells on glass dishes with the same cultural medium. These findings suggested that the lower concentration FBS of medium should be used for researching the effect of extracellular matrix on cells, and that the ABM is idealer matrix of culture for inducing the differentiation of carcinoma cells in vitro.

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What this paper is about

Artificial basement membrane (ABM) with spongiform configuration was mainly made of type I collagen (rat tail) which was formed into hydrated collagen lattices (HCLS), fibronectin, type IV collagen and laminin. ABM could cut off 10% of the fetal bovine serum (FBS) in medium, raise the viability of cells and prolong the period of subculture. The growth effecien-cy, plating effeciency and the rate of clonal growth of CNE-2 cells propagated on ABM with 2-5% FBS cultural medium were higher but the uptake of 3H-TdR was lower than that of these cells on glass dishes with the same cultural medium. These findings suggested that the lower concentration FBS of medium should be used for researching the effect of extracellular matrix on cells, and that the ABM is idealer matrix of culture for inducing the differentiation of carcinoma cells in vitro.

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Available abstract

Artificial basement membrane (ABM) with spongiform configuration was mainly made of type I collagen (rat tail) which was formed into hydrated collagen lattices (HCLS), fibronectin, type IV collagen and laminin. ABM could cut off 10% of the fetal bovine serum (FBS) in medium, raise the viability of cells and prolong the period of subculture. The growth effecien-cy, plating effeciency and the rate of clonal growth of CNE-2 cells propagated on ABM with 2-5% FBS cultural medium were higher but the uptake of 3H-TdR was lower than that of these cells on glass dishes with the same cultural medium. These findings suggested that the lower concentration FBS of medium should be used for researching the effect of extracellular matrix on cells, and that the ABM is idealer matrix of culture for inducing the differentiation of carcinoma cells in vitro.

Key concepts: Fibronectin, Laminin, Basement membrane, Fetal bovine serum, Type IV collagen, Subculture (biology), Extracellular matrix, Biology

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EFFECT OF ARTIFICIAL BASEMENT MEMBRANE ON THE GROWTH OF NASOPHARYNGEAL CARCINOMA CELL LINE (CNE-2) — Research Paper | ScholarLens