The Inhibitory Activity of Flos caryophylli and Rheum officinale on Vibrio harveyi and Its Biofilm

[Objective]To observe the inhibitory effects of Rheum officinale and Flos caryophylli on Vibrio harveyi and its biofilm. [Method]The inhibitory effects of Rheum officinale and Flos caryophylli on Vibrio harveyi were tested by disc agar diffusion,and the MIC and MBC were detected by the double dilution; the effects of Rheum officinale and Flos caryophylli on the formation of Vibrio harveyi biofilm were measured by MTT assay. [Result]Rheum officinale had inhibitory effects with the diameter of inhibition zone( DIZ) of( 15. 31 ± 0. 4) mm,and the MIC and MBC were 7. 183 mg /ml; Flos caryophylli had inhibition effects with DIZ of( 11. 53 ± 0. 6) mm,and the MIC and MBC were 15. 625 mg /ml,62. 5 mg /ml. Both Rheum officinale and Flos caryophylli exhibited a potent inhibitory activity against Vibrio harveyi biofilm formation( P 0.01). [Conclusion]Rheum officinale and Flos caryophylli could significantly inhibit Vibrio harveyi and its biofilm.