Effects of aspirin on endothelial progenitor cell function and its induced nitricoxide synthase

Objective:To investigate whether aspirin had influences on endothelial progenitor cells(EPCs).Methods:Total mononuclear cells(MNCs) were isolated from peripheral blood by ficoll density gradient centrifugation,and then the cells were plated on fibronectin-coated culture dishs.After 7 days cultured,attached cells were stimulated with aspirin(to make a series of final concentrations:1,2,5,10mmol/L)for 3,6,12,24h.EPCs were characterized as adherent cells double positive for DiLDL-uptake and lectin binding by direct fluorescent staining.EPCs proliferation,migration were assayed with MTT assay and modified Boyden chamber assay,respectively.EPCs adhesion assay was performed by replating those on fibronectin-coated dishes,and then adherent cells were counted.In vitro vasculogenesis activity was assayed by in vitro vasculogenesis kit.iNOS was assayed by western blot.Results:Incubation of isolated human MNCs with aspirin decreased the number of EPCs,aspirin also decreased EPCs proliferative,migratory,adhesive,in vitro vasculogenesis capacity and iNOS in a concentration and time dependent manner.Conclusion:Aspirin decrease the number,proliferative,migratory,adhesive and in vitro vasculogenesis capacity of EPCs,aspirin also decrease iNOS in EPCs.