Effects of gypenosides on expression of Jun protein in hippocampus of rats with cerebral ischemia-reperfusion injury

Objective To study the inhibitive effect of gypenosides on expression of immediate early gene c-jun in hippocampus of rats with cerebral ischemia-reperfusion injury.Methods Modified Pulsinelli 4vessel occlusion(4-VO) was used to develop the rat model of acute cerebral ischemia-reperfusion.Brain specimens were taken 6 h,24 h,and 72 h after cerebral ischemia-reperfusion injury.ABC immunohistochemical anslysis was carried out to observe the expressions of Jun protein at different times in hypocampus of rats receiving high and low pocampus dosage GP.Results The expression of Jun protein was highes in the model group,compared with the blank group.The expression of Jun protein in hippocampus of experimental groups decreased progressively with increasing time of reperfusion(6 h24 h72 h).The expression of Jun protein in the groups of reperfusion 6 h ranked in the following order: model group high GP dosage group low GP dosage group.Compared with model group,high and low dosage GP groups showed noticeable inhibitive effect on expression of Jun protein in hippocampus at different cerebral ischemia-reperfusion times.Conclusions GP down-regulates the expression of c-jun and inhibits the apoptosis of nerve cells,thus preventing cerebral injury in ischemia-reperfusion rats,and improving the function of impaired nerves;the protective role is related with the dosage of GP.