The Role of Colquhounia Root Tablet against Acute Lung Injury Oleic Acid Pretreated in Rats

Objective To explore the protective effects of colquhounia root on rats with acute lung injury induced by oleic acid. Methods 45 healthy SD rats were randomly divided into three groups: the control group( N group),the ALI group and the colquhounia root treated group( C group) so 15 rats in each group. In N group,sodium chloride 2mL were instilled into the stomach of each rat for ten consecutive days before intravenous injection of sodium chloride 0. 04mL·kg-1. In ALI group,sodium chloride 2mL were instilled into the stomach of each rat for ten consecutive days until oleic acid 0. 04mL·kg-1was injected into trail vein to establish ALI models,and in C group,colquhounia root talet with 600mg·( kg·d)-1were instilled into the stomach of each rat for ten consecutive days before intravenous injection of same dose oleic acid with the ALI group. After 4h of establishing model,all animals were sacrificed to collect lung tissue of rats to test lung tissue wet / dry weight ratio( W / D),collect rat blood serum,bronchoalveolar lavage fluid to test lung permeability index( LPI). Lung tissue was fixed-embedded to do hematoxylin-eosin( HE) staining to test lung injury score( LIS). Results Through general observation,the lung tissue was normal in N group. In ALI group, the lung tissue swelled,and there were widespread bleeding points on the surface. In C group,lung tissue swelled slightly. Under the microscope,alveolar epithelium was integrity,and there was no significant pathological change in alveolar spaces and pulmonary interstitium in N group. In ALI group,the alveolar wall was not complete. There were lots of inflammatory cells infiltration,red blood cell exudation and alveolar interstitial edema. Change in C group was not serious. The LIS of ALI group was significantly increased than that of N group( P 0. 01). The LIS of C group was significantly lower than that of ALI group( P 0. 01),but still higher than that of N group( P 0. 05). In ALI group,W / D and LPI were respectively significantly increased than that of N group and C group( P 0. 05). In C group,W / D was higher than that of N group( P 0. 01), but there was no significant difference between that of C group and N group. Conclusion The colquhounia root can protect rats from oleic acid induced acute lung injury.