Assessment of therapeutic effect and safety by dynamic monitoring B-type natriuretic peptide and electrocardiogram during perioperation of cellular transplantation for acute myocardial infarction rats

Objective To investigate the efficacy and safety of dermal fibroblasts(DFs) of transplantation in situ at myocardium infarct sites for repairing infarct myocardium in rats after acute myocardial infarction(AMI),after the DFs were transfected by both myogenic transcription factor——myogenic determination(MyoD) gene and gap junctions protein-connexin43(Cx43) gene.Methods Sixty male Sprague-Dawley(SD) rats were randomly divided into 4 groups.normal control group: 100 μl cell culture mediums were injected into the normal myocardial tissues.myocardial infarction control group: 100 μl cell culture mediums were injected into the scar tissues of the myocardial infarction rats.normal transplantation group: 100 μl cell suspension of MyoD/Cx43-converted DFs(2.0×106 cells/ ml) which labeled by bromodeoxyuridine(BrdU) were injected into the normal myocardial tissues.myocardial infarction transplantation group: 100 μl cell suspension of MyoD/Cx43-converted DFs(2.0×106 cells/ml) with BrdU labeled were injected into the scar tissues after myocardial infarction.The changes of dynamic electrocardiogram(ECG) and plasma B-type natriuretic peptide(BNP) concentration were measured before and 4 weeks after operation of cell transplantation.Results After MyoD/Cx43-converted DFs were transplanted into normal myocardial tissue and scar tissue,plasma BNP concentration in myocardial infarction control group and myocardial infarction transplantation group were higher than those in normal control group and normal transplantation group(P0.01),and plasma BNP concentration in myocardial infarction control group were higher than those in myocardial infarction transplantation group after 4 weeks(P0.01).QRS of leadⅡwave was broadened and ST segment shifted up in the AMI rat models.The fall of ST segment and T wave was more than 50% within 2 weeks after cell transplantation.There was no serious arrhythmia in both normal transplantation group and myocardial infarction group.Moreover,compared with myocardial infarction control group,QRS wave become more narrow,R wave was relatively increased and ST segment shifted back to baseline after cell transplantation in the myocardial infarction transplantation group and myocardial infarction control group.Conclusion ECG should be improves and plasma BNP concentration be decreases after MyoD/Cx43-converted DFs was transplanted into myocardial scar tissue of AMI rats.Both BNP and ECG dynamic monitor during perioperation of cell transplantation can be used as a reliable technique to evaluate the effect of cellular cardiomyoplasty.