Study of extracellular release of HMGB1 and its mechanism in lipopolysaccharide-induced bladder cancer T24 cells

Objective To investigate the extracellular release of high mobility group box 1(HMGB1) in bladder cancer T24 cells induced by lipopolysaccharide(LPS) and its possible mechanism.Methods The changes of HMGB1 concentration in the culture medium and HMGB1 mRNA expression were investigated after T24 cells were stimulated with 100 μg/L LPS for different hours.The HMGB1 concentration and the level of HMGB1 mRNA expression were deter mined by ELISA and real-time fluorescent quantitative PCR,respectively.Inhibitory effect of PI3K/AKT pathway in hibitor was observed on HMGB1 release.Results The HMGB1 concentration in the culture medium and the level of HMGB1 mRNA expression significantly increased in a time-dependent manner after T24 cells after induced by LPS for 12 h.LY294002 partly inhibited extracellular release of HMGB1 in LPS-induced T24 cells.Conclusion LPS induces bladder cancer cells to release HMGB1,which may be related to PI3K/AKT signaling pathway.