Effect of Different Dose Ulinastin On Acute Lung Injury Induced By High Temperature Gas In Guinea Pigs

Objective To study the effect of different dose ulinastatin on acute lung injury induced by high temperature gas in guinea pigs.Methods 40 male guinea pigs were randomly divided into 4 groups: the control group(N),the injury group(I),the ulinastatin 100000 U/kg group(A),ulinastatin 1000000 U/kg group(B),I,A,B group were made into the model of acute lung injury by inhaling high-temperature gas,and then the A,B group were injected with different dose ulinastatin.Measuring the TNF-α and neutrophil elastase content with ELLSA method after 4 hours,simultaneously measuring lung wet /dry weight ratio(W/D) and arterial blood gas analysising,another HE staining of lung tissue,observing the pathological changes of lung tissue by light endoscopic.Results In A,B group,the serum TNF-α and neutrophil elastase contents,lung W/D,pathological changes were lower than those in I group;there were significant differences between I and A,B group in the serum TNF-α and neutrophil elastase contents,lung W/D and pathological changes and there were also differences between A and B.Conclusion Ulinastatin reduces acute lung injury inflammatory mediators levels,improves the lung ventilation and pathological changes and protects the function of the lung tissue gradually with ulinastatin dose increasing.