ISSR analysis on genetic diversity between the wild and cultured populations of Anabarilius grahami

We assessed the genetic diversity and genetic structure between wild and cultured populations of this species using ISSR PCR(13 primers).Ninety-seven bands were amplified and 72 bands displayed polymorphic.The genetic diversity of the wild population was higher(PPB=62.89%;H=0.249 0;I=0.363 6) than that of the cultured population(PPB=58.76%;H=0.226 7;I=0.331 4).In total,the genetic diversities of both populations were in high levels.The Gst value based on Nei's genetic diversity analysis was 0.082 6,while Fst value resulted from AMOVA was 0.079 6.Both the two statistics revealed that there were about 8% genetic differentiation existed between the two populations,that was,most genetic differentiation occurred within the populations.UPMGA cluster showed that the individuals of the two populations were mostly assembled respectively,and then mixed each other.the Nm value(5.55) was high.These results indicated that there were some degree of genetic differentiation between wild and cultured populations,but the genetic variation did not reach to the level of the populations,there were certain communication spaces between the two populations.