Effects of menotrophin in-vitro intervention on grafts follicles before mice ovary autotransplantation and allotransplantation

Objective To investigate the effects of menotrophin in-vitro intervention on survival grafts ovarian follicles in mouse half ovary transplantation.Methods ICR mice were randomly divided into five groups:(1)fresh ovarian tissue autotransplantation group(FTG).Ovaries were autotransplanted under the renal capsule after excised.(2)autotransplantation with intervention group(AUHIG).Ovaries were autotransplanted under the renal capsule after cultured for 3 hours in the medium contain with menotrophin.(3)autotransplantation without intervention group(AUHNG).Ovaries were autotransplanted under the renal capsule after cultured for 3 hours in the medium without menotrophin.(4) allotransplantation with intervention group(ALHIG).Ovaries were allotransplanted under the renal capsule after cultured for 3 hours with menotrophin.(5)ovariectomized control group(OVXG).The survival rate and the number of normal ovarian follicles of graft were observed at 3 days after operation.At a month after operation,the recovery of estrous cycle and the development of the graft were observed,and the levels of follicle-stimulating hormone(FSH) and estradiol were detected.Results The number of survival follicles in FTG and AUHIG was significantly higher than that of AUHNG and ALHIG at 72 h after transplantation(all in P0.01).The recovery of estrous cycle were 100% in FTG,AUHIG and AUHNG,which were significantly higher than that in ALHIG with 67%(all in P0.05).And the recovery time of estrous cycle was shortest in FTG and AUHIG.The number of follicles in AUHIG was more than that in AUHNG,but less than that in FTG.Compare with OVXG,the levels of FSH were lower and the levels of estradiol were higher in the other 4 groups(all in P0.01).And the levels of estradiol in FTG and AUHIG were higher than that in AUHNG and ALHIG(all in P0.01).Conclusions The menotrophin intervention of transplant ovary in-vitro culture can increase the survival rate of transplant follicle,which is an effective way to improve the function of ovary transplantation.