Preliminary study of Linum usitatissmum seed irradiated by ~(12)C~(6+) heavy ions

The study of heavy ion beams as a new irradiation resource on seeds of linseed (Linum usitatissmum L.) was reported. After irradiated by 12C 6+ heavy ions (with irradiation dose: 6×108 cm-2,1.8×109 cm-2 and 3.6×109 cm-2 respectively),the biological characters,agricultural traits,pollens and polymorphic DNA of sample was studied in Linum usitatissmum L.. The results showed that 6×108 cm-2 of 12C 6+ could promote the linseed germination rate,plant height and pollen viability. The 1000-seed weight and the oil content of seed irradiated by 12C6+ heavy ions was increased with augment of irradiation dose respectively. It has been found that 3.6×109 cm-2 is the most effective dose for increment both of seed weight and oil content with 16.5% and 19.9%,respectively. Cytological observation re-veals that the highest dose (3.6×109 cm-2) can also induce alteration of pollen shape of linseed. Furthermore,RAPD(Randomly amplified polymorphic DNAs) analysis shows that 99 DNA fragments are amplificated by 14 primers and the polymorphic ratio is 52.5%.