Study of fermentation condition ofpectinase-producing strain HDYM-02

A pectinase-producing bacterial strain HDYM-02 was separated from the liquid of flax retting in BaYan Flax Company.Strain HDYM-02 was identified as Bacillus cereus according to the test of morphological,physiological,biochemical characteristics and analysis of 16S rDNA sequencing.The single factor test on fermentation condition indicated that strain HDYM-02 showed a high pectinase activity when fermentation temperature was 33℃,urea as nitrogen,initial pH value was 7.0 and concentration of NaCl was 0.5-0.7%.The three main factors affecting reducing sugar production were fermentation temperature,concentration of urea and initial pH value.L9(33) orthogonal test manifested that the optimum pectinase-producing condition of strain HDYM-02 was fermentation temperature 35℃,concentration of urea 0.5% and initial pH value 6.5.