Effects of Excessive Iodine on Apoptosis in Primary Cultured Cortical Neurons of Rats

Objective To investigate the effects of excessive iodide on apoptosis in primary cultured cortical neurons of rats. Methods The primary cultured cortical neurons of rats were exposed to different concentrations of KI.The apoptosis was observed by AO-stained fluorescin,the apoptosis rate by propidium iodide-stained flow cytometry,the content of NO and NOS by spectrophotometry. ResultsExcessive iodine may induce apoptosis in the primary cultured cortical neurons of rats and the apoptosis rates increased gradually with the concentrations of KI.Compared with the control group,the content of NO and the activity of NOS in experimental groups were higher.Conclusions Excessive iodide may induce the apoptosis in the primary cultured cortical neurons of rats and the apoptosis is concentration-dependent.The mechanism of apoptosis may be related with the increase of NO and NOS.