PROTECTIVE EFFECT OF PENTOXIFYLLINE ON LIVER AND KIDNEY IN RAT MODEL OF OBSTRUCTIVE JAUNDICE

ObjectiveTo study the protective effects of pentoxifylline on liver and kidney of obstructive-jaundice model in rat.MethodsSeventy-seven rats were randomly divided into three groups: sham-operated group (n=7); common bile duct ligation group(BDL group, n=35); anti-TNF-α group (PTX group, n=35). The model of obstructive jaundice was established in BDL group and PTX group. The rats in the PTX group received 5 g/L pentoxifylline 1 mL/100 mg per rat intraperitoneally every day after operation. Both groups were randomly divided into five sub-groups, with seven rats in each sub-group. Serum ALT, TNF-α, Cr, and TB were determined at 1st, 4th, 7th, 10th, and 14th day respectively after operation. The kidney and liver were observed pathologically at 14th day after operation in both BDL group and PTX group.ResultsSerum TNF-α in BDL group at 14th day after operation was significantly higher than that in the sham-operated and PTX groups (F=6.33, q=3.18, 4.97, P0.01).Serum ALT showed positive correlation with TNF-α in BDL group(r=0.758, P0.01), serum Cr correlated positively with TNF-α in BDL group(r=0.502, P0.05).Serum ALT and TNF-α were well correlated (r=0.835, P0.01) and serum Cr and TNF-α were not correlated in PTX group. Microscopic examination showed that liver cell necrosis was more in BDL group than in PTX group. Bile was filled up in the bile duck in both groups. Renal tubular epithelium was swelling. ConclusionSerum TNF-α rises in rats with obstructive jaundice. Pentoxifylline given intraperitoneally can protect the function of the liver but not of the kidney in rat model of obstructive jaundice.