Protective Effects of Oleanolic Acid on Oxidative Damaged Human Umbilical Vein Endothelial Cells

OBJECTIVE:To study the protective effects of oleanolic acid(OA)oxidative damaged human umbilical vein endothelial cells(HUVECs). METHODS:HUVECs were cultured with DMEM high glucose medium of 10% serum [containing ox-LDL0,25,50,100,200 and 400 μg/ml];the activity of cells was detected by CCK-8 assay to screen optimal concentration of medium.HUVECs were cultured with OA(0,10,20,40,60,80 and 100 μmol/L),and the activity of cells was detected by CCK-8 assay to screen optimal concentration of OA. The oxidative damaged HUVECs(induced by 100 μg/ml ox-LDL) were treated with OA(5,10,20,40 μmol/L),and the activity of cells was detected by CCK-8 assay;the levels of NO,NOS,CAT and GSH-PX were detected. RESULTS:For inducing model,the optimal concentration of ox-LDL was 100 μg/ml,and that of OA was 0-40 μmol/L;5,10,20,40 μmol/L OA enhanced the survival rate of HUVECs,enhanced the activities of CAT,GSH-PX and NOS and increased NO levels. CONCLUSIONS:OA can protect the ox-LDL oxidative damage HUVECs,and the protective mechanism may be associated with the improvement of CAT,GSH-PX,NOS activity.