Expression of recombinant plasmid pCS2-MT/Axin and pEGFP-N3/APC in SW480 colon carcinoma cell line

【Objective】To express Axin gene and APC gene fragment in colon cancer cell SW480. 【Method】 The recombinant plasmid pCS2-MT/Axin and pEGFP-N3/APC5 was identified by double digested. Plasmid was cotransfected into SW480 cell line by liposome. The target gene Axin and APC were identified by reverse transcription -polymerase chain reaction (RT-PCR), and the label protein GFP and MYC were identified by western blotting. 【Result】 The agarose gel elctrophrosis showed a 4.3 kb and a 2.5 kb band of the digestion product of pCS2-MT/ Axin, a 4.7 kb and a 2.0 kb band of pEGFP-N3/APC5, which matched the expected size. The target genes can be detected by RT-PCR,and the label proteins can be detected by western blotting. 【Conclusion】 The recombinant plasmid pCS2-MT/Axin and pEGFP-N3/APC5 could be expressed successfully in SW480 cell line .It may provide an experimental basis for the further study of their function in cells.