Effect of Propofol on Expression of HMGB-1 in Rats with Acute Lung Injury

Objective To investigate the protective effect of propofol on lung and its mechanism of action.Methods The endotoxin was injected into rats via the caudal vein to induce acute lung injury(ALI).The propofol and zinc protoporphyrin were also given via tail vein injection. The Wistar rats were randomly divided into three groups:control group(group C),lipopolysaccharide(LPS)group(group L),and LPS+propofol group(group LP).Saline solution was injected into rats of control group via caudal vein;lipopolysaccharide(7.5mg·kg-1)was injected into rats of LPS group via caudal vein;lipopolysaccharide(7.5mg·kg-1)and propofol(30mg· kg-1)were injected into rats of LPS+propofol group via caudal vein at the same time.The PaO2 was measured before and at 3,6and 12hours after treatment.Lung wet/dry weight ratio,lung injury score and high mobility group box-1(HMGB-1)expression were measured after the experiment.Results There were no significant differences in the PaO2among the three groups before the experiment.After LPS injection,PaO2in group L continuously decreased and was significantly lower than that in group C(P0.05).After the experiment,lung wet/dry weight ratio,lung injury score and HMGB-1content in group L were significantly increased as compared with group C(P0.05).Compared with group L,the PaO2 was obviously improved and lung wet/dry weight ratio,lung injury score and HMGB-1content were significantly decreased in group LP(P0.05). Conclusion Propofol can protect lung from endotoxin-induced acute lung injury through inhibiting the excessive expression of HMGB-1.