Expression of Truncate HIV-1 Envelope Glycoprotein gp160 in Saccharomyces cerevisiae

The coding sequence of truncate gp160 was amplified by polymerase chain reaction(PCR) and cloned into yeast expression vector YEpFLAG-1 to construct plasmid of YEp-gp160Δ12.The plasmids were transformed into yeast Bj3505,and the recombinant strains were identified by cultivating in SC media with-out tryptophan.After induction in YP media,the whole proteins of recombinant cells were analyzed by SDS-PAGE and Western blotting,and the high-yielding cells were selected.The ELISA performed with a panel of human sera suggested that the purified recombinant gp160Δ12(rgp160Δ12) has good bioactivity.