Effects of Se-SMC, Sodium Selenite and VE and Combination of Se-SMC and VE on Antioxidant Defense System in Rats

Objective: To compare the effects of organic selenium such as L-Se-methylselenocysteine(Se-SMC) and inorganic selenium(sodium selenite) on antioxidant functions in rats,and explore the synergistic effect of Se-SMC and vitamin E(VE) on antioxidant functions invivo.Methods: Adult female rats were randomly divided into five groups including blank group,organic selenium group,inorganic selenium group,VE group,and organic selenium/VE combination group.The rats were administrated with designed drugs by gavage for 30 d.The serum levels of malonaldehyde(MDA) content,total antioxidant activity(T-AOC) and superoxide dismutase(SOD) activity in the rats were determined at 10,20 d and 30 d,respectively.After 30 d,the rats were sacrificed,and MDA,glutathione(GSH) and protein carbonyl(CP) contents,and T-AOC level,as well as SOD and glutathion peroxidase(GSH-Px) activities were determined in the heart,liver and kidney of the rats.Results: SeSMC resulted in higher GSH-Px activity than inorganic selenium in rat tissues.The combination of organic selenium and VE signi cantly enhanced T-AOC and SOD activities in the serum of rats after administration for 20 d and 30 d and elevated T-AOC and SOD activities as well as GSH-Px and GSH contents in the heart,liver and kidney of rats.Conclusion: Organic selenium is better than inorganic selenium for enhancing GSH-Px activity in heart,liver and kidney tissues.In addition,the combination of organic selenium and VE can enhance antioxidant functions in rats.