STUDY ON PHELLINUS IGNIARIUS FERMENTOR MAGNIFICATION CULTURE

The paper compared Phellinus igniarius shake flask culture with fermenter culture at different stages of the physiological indices,it showed that: shake flask culture in conditions that the inoculum was 10%,the temperature was 28 ℃ and the speed was 160 r/min,make the mycelium volume in the 168 h reached maxmium,reducing sugars and amino nitrogen in the 120 h and 72 h respectively,reached a peak,and the optimal pH value was 5.5;fermenter culture in conditions that the liquid volume was 5 L,the inoculum was 10%,stirring speed was 200 r/min and ventilation was 1∶1(v/v),the mycelium volume in the 120 h reached the maxmium,reducing sugar and amino nitrogen content declined with the growth of Phellinus,and the optimum fermentation pH value was 5.5.There is significantly difference between fermenter culture and shake flask culture,and to master the law of fermenter culture can realized large-scale production of Phellinus igniarius.