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2001•Journal of Nanchang UniversityRequires access

MECHANISM OF ETHIDIUM BROMIDE FLUORESCENCE ENHANCEMENT IN ORGANIC SOLVENT AND ON BINDING TO DNA

Huang Wei

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Abstract

The effect of organic solvents on ethidium bromide(EB) and the complex of DNA-EB were studied by spectrofluorimetry,the mechanism of EB fluorescence enhancement in organic solvents and on binding to DNA has been investigated.From an examination of the effect of different solvents on the fluorescence intensity and fluorescent spectra of EB and DNA-EB complex,we concluded that the viscosity,polarity,hydrophobic and the capacity of capturing proton of solution play important roles in the fluorescence enhancement of EB.While the quenching of fluorescence of DNA-EB complex in large weight fraction of solvent is primarily responsible for the conformational change of DNA which exclude the EB molecules from the interior of DNA.

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What this paper is about

The effect of organic solvents on ethidium bromide(EB) and the complex of DNA-EB were studied by spectrofluorimetry,the mechanism of EB fluorescence enhancement in organic solvents and on binding to DNA has been investigated.From an examination of the effect of different solvents on the fluorescence intensity and fluorescent spectra of EB and DNA-EB complex,we concluded that the viscosity,polarity,hydrophobic and the capacity of capturing proton of solution play important roles in the fluorescence enhancement of EB.While the quenching of fluorescence of DNA-EB complex in large weight fraction of solvent is primarily responsible for the conformational change of DNA which exclude the EB molecules from the interior of DNA.

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Available abstract

The effect of organic solvents on ethidium bromide(EB) and the complex of DNA-EB were studied by spectrofluorimetry,the mechanism of EB fluorescence enhancement in organic solvents and on binding to DNA has been investigated.From an examination of the effect of different solvents on the fluorescence intensity and fluorescent spectra of EB and DNA-EB complex,we concluded that the viscosity,polarity,hydrophobic and the capacity of capturing proton of solution play important roles in the fluorescence enhancement of EB.While the quenching of fluorescence of DNA-EB complex in large weight fraction of solvent is primarily responsible for the conformational change of DNA which exclude the EB molecules from the interior of DNA.

Key concepts: Ethidium bromide, Fluorescence, Chemistry, DNA, Solvent, Quenching (fluorescence), Photochemistry, Fluorescence anisotropy

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