Effects of Interleukin-1βand tumor necrosis factor-αon transcription of matrix metalloproteinase-28 in lumbar intervertebral disc

Objective To investigate whether matrix metauoproteirase-28(MMP-28) can be regulated by interleubin-β(IL-1β) and tumor necrcsis factor-α(TNF-α) in human nucleus pulposus cells after coculture. Methods After the third generation of human nucleus pulposus cells were stimulated with different concentration of IL-1β(0 ng /mL,10 ng /mL,50 ng /mL)and TNF-α(0 ng /mL,50 ng /mL,100 ng /mL) 72 h in vitro,MMP-28 mRNA was quantified by RT-PCR,the difference between each concentration were compared by one-way analysis of variance. Results There was no sign indicated that the various concentration of IL-1β(0 ng /mL,10 ng /mL,50 ng /mL) can affect human nucleus pulposus cells MMP-28 transcription(0. 325 ± 0. 019 vs. 0. 343 ± 0. 018 vs. 0. 343 ± 0. 018,P 0. 05). But cytokines TNF-α could induce the expression of MMP-28,various concentration groups present significant difference(0. 325 ± 0. 019 vs. 0. 515 ± 0. 02 vs. 0. 610 ± 0. 012,P 0. 01),and with the concentration increasing,the effect was more prominent(0. 610 ± 0. 012 vs. 0. 515 ± 0. 02,P 0. 01). Conclusion IL-1β doesn't affect human nucleus pulposus cells MMP-28 transcription in vitro. However,TNF-α can up regulate the MMP-28 mRNA of the nucleus pulposus cells and the effects are strengthened by enhancing concentration.