Production of HPV16/18/33/58 Major Capsid Protein L1 with Baculo virus Expression System

Objective: To obtain human papillomavirus(HPV) type 16/18/33/58 major capsid protein L1 with bac ulovirus expression system.Methods: The genes encoding the L1 proteins of HPV 16/18/33/58 serotype were cloned and modified by a variety of strategies to HPV16/18/33/58 mL1.The modified genes were inserted into the pFastBac Dual vector,and the recombinant bacmid was generated after the E.coli DH10Bac competent cell was transformed by the recombinant pFastBac Dual vector,recombinant baculovirus were transfected into insect cell Sf9.Results: Recombinant baculovirus expressing mL1 protein of HPV16/18/33/58 serotypes was obtained.Western blotting and SDS-PAGE analysis showed that the mL1 protein of the HPV strains was expressed when the Sf9 cells were infected by the recombinant baculovirus,and the mL1 proteins were found distributed mainly in the cell.The mL1 was highly expressed by optimized expression time and multiplicity of infection(MOI).Conclusion: Cloning and expression of L1 protein of multiple subtypes of HPV,laid the foundation for the Chinese indepen dent serotype vaccine.