2012•Zhongguo shiyan fangjixue zazhiRequires access
Simultaneous Determination of Jatrorrhizine,Epiberberine,Coptisine,Palmatine and Berberine Hydrochloride in Purification Parts of 'Sanrelun' Compound Decoction by HPLC
Zhao Mian
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Abstract
Objective:To determination of jatrorrhizine,epiberberine,coptisine,palmatine and berberine hydrochloride in purification parts of 'Sanrelun' compound.Method: 'Sanrelun' compound through HPD 400 macroporous resin with 10%,30%,60%,90% ethanol eluted,then was determinated the five alkaloids in four purification parts by HPLC.Result: The highest content of alkaloids was the part of 30%EtOH purified by HPD 400 macroporous resin.Conclusion: In this study,the method of macroporous resin purification is simple,alkaloid content which was determinated by HPLC is highest in the part of 30% of the purified,which provide the foundation for effective parts selection.