Effects of Galectin-3 small interfering RNA on the expressions of Cyclin D1,p21,p27 protein and the effect on cell proliferation of Eca109 esophageal carcinoma cell line

Objective To observe the changes of cell proliferation activity and expressions of Galectin-3,CyclinD1,p21 and p27 protein in Eca-109 cell line after transfected by Galectin-3 small interfering RNA(siRNA).Methods The Galectin-3 siRNA chain was constructed and transfected into Eca-109 cell line by lipofectamine 2000,then Real-time PCR,MTT,Flow cytometry and immuocytochemistry were respectively used to detect the transfection efficiency of siRNA,the cell proliferation activity,cell cycle distribution and the expression levels of Galectin-3,Cyclin D1,p21 and p27protein in Eca-109 cell line.Results The cell proliferation activity of Eca-109 cell line was significantly decreased,and cell distribution was mainly at G1 stage,and the expression levels of Galectin-3 and Cyclin D1 were decreased,however,the expression levels of p21 and p27 protein were increased.Conclusion The application of siRNA can effectively inhibit the expression of Galectin-3 and cell proliferation activity of Eca-109 cell line,and Galectin-3 is involved in the proliferation of Eca-109 cell line and is closely correlated with the expressions of Cyclin D1,p21,p27 protein,which may become the target gene for treating esophageal cancer.