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Effects of Astragalus Injection on the Expression of p38 Mitogen-activated Protein Kinase in Asthmatic Rats

Huang Cui-ping

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Abstract

Objective To explore the effect of astragalus injection on the expression of p38 mitogen-activated protein kinase(p38 MAPK) in asthmatic rats.Methods Ovalbumin(OVA) was injected intraperitoneally and inhaled to produce the asthmatic model.Twenty-four rats were randomly divided into three groups: control group,asthma group and astragalus group.The concentration of IL-5 in BALF and the expression of phospho-p38 MAPK in lung tissue were measured by ELISA and Western blot,respectively.Results The expression of phospho-p38 MAPK was up-regulated in the lung tissue of asthmatic rats.Compared with asthma group,astragalus injection significantly decreased the expression of phospho-p38 MAPK,the concentration of IL-5 and the number of eosinophil in BALF(P0.01),and alleviated the histopathological damage of lung tissue.There were positive correlations between the expression of phospho-p38 MAPK and the concentration of IL-5 and the number of eosinophil in BALF.Conclusion p38 MAPK may play a role in pathological process of asthma.Astragalus could effectively treat asthma by inhibiting the expression of p38 MAPK.

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What this paper is about

Objective To explore the effect of astragalus injection on the expression of p38 mitogen-activated protein kinase(p38 MAPK) in asthmatic rats.Methods Ovalbumin(OVA) was injected intraperitoneally and inhaled to produce the asthmatic model.Twenty-four rats were randomly divided into three groups: control group,asthma group and astragalus group.The concentration of IL-5 in BALF and the expression of phospho-p38 MAPK in lung tissue were measured by ELISA and Western blot,respectively.Results The expression of phospho-p38 MAPK was up-regulated in the lung tissue of asthmatic rats.Compared with asthma group,astragalus injection significantly decreased the expression of phospho-p38 MAPK,the concentration of IL-5 and the number of eosinophil in BALF(P0.01),and alleviated the histopathological damage of lung tissue.There were positive correlations between the expression of phospho-p38 MAPK and the concentration of IL-5 and the number of eosinophil in BALF.Conclusion p38 MAPK may play a role in pathological process of asthma.Astragalus could effectively treat asthma by inhibiting the expression of p38 MAPK.

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Available abstract

Objective To explore the effect of astragalus injection on the expression of p38 mitogen-activated protein kinase(p38 MAPK) in asthmatic rats.Methods Ovalbumin(OVA) was injected intraperitoneally and inhaled to produce the asthmatic model.Twenty-four rats were randomly divided into three groups: control group,asthma group and astragalus group.The concentration of IL-5 in BALF and the expression of phospho-p38 MAPK in lung tissue were measured by ELISA and Western blot,respectively.Results The expression of phospho-p38 MAPK was up-regulated in the lung tissue of asthmatic rats.Compared with asthma group,astragalus injection significantly decreased the expression of phospho-p38 MAPK,the concentration of IL-5 and the number of eosinophil in BALF(P0.01),and alleviated the histopathological damage of lung tissue.There were positive correlations between the expression of phospho-p38 MAPK and the concentration of IL-5 and the number of eosinophil in BALF.Conclusion p38 MAPK may play a role in pathological process of asthma.Astragalus could effectively treat asthma by inhibiting the expression of p38 MAPK.

Key concepts: Eosinophil, p38 mitogen-activated protein kinases, Ovalbumin, Astragalus, MAPK/ERK pathway, Protein kinase A, Western blot, Medicine

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