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Protection in vitro of Epigallocatechin Gallate on Oxidative Stress Induced by Influenza Infection

Yang Zhan-qiu

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Abstract

Objective:To investigate the molecular mechanism of the anti-influenza A virus effects of epigallocatechin gallate(EGCG) derived from green tea in vitro.Method:The cell viability of EGCG was determined by using MTT assay.And virus inhibitory rate was evaluated based on the observation of cytopathic effect(CPE),hemagglutination assay and Real-time Quantitative PCR(QPCR).The mechanisms of action for the observed sensitivity of influenza A to EGCG at the molecular level was assessd by confocal microscope and flow cytometry.Result: Exposing Madin-Darby canine kidney(MDCK) cells with 20 mg · L-1 EGCG could inhibit influenza A replication in a time-dependent manner,probably by interrupting postbinding step.EGCG treatment could mitigate oxidative stress in influenza-infected cells and reduce the apoptosis induced by influenza infection.Conclusion:EGCG inhibits oxidative stress and apoptosis induced by influenza virus infection.

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What this paper is about

Objective:To investigate the molecular mechanism of the anti-influenza A virus effects of epigallocatechin gallate(EGCG) derived from green tea in vitro.Method:The cell viability of EGCG was determined by using MTT assay.And virus inhibitory rate was evaluated based on the observation of cytopathic effect(CPE),hemagglutination assay and Real-time Quantitative PCR(QPCR).The mechanisms of action for the observed sensitivity of influenza A to EGCG at the molecular level was assessd by confocal microscope and flow cytometry.Result: Exposing Madin-Darby canine kidney(MDCK) cells with 20 mg · L-1 EGCG could inhibit influenza A replication in a time-dependent manner,probably by interrupting postbinding step.EGCG treatment could mitigate oxidative stress in influenza-infected cells and reduce the apoptosis induced by influenza infection.Conclusion:EGCG inhibits oxidative stress and apoptosis induced by influenza virus infection.

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Available abstract

Objective:To investigate the molecular mechanism of the anti-influenza A virus effects of epigallocatechin gallate(EGCG) derived from green tea in vitro.Method:The cell viability of EGCG was determined by using MTT assay.And virus inhibitory rate was evaluated based on the observation of cytopathic effect(CPE),hemagglutination assay and Real-time Quantitative PCR(QPCR).The mechanisms of action for the observed sensitivity of influenza A to EGCG at the molecular level was assessd by confocal microscope and flow cytometry.Result: Exposing Madin-Darby canine kidney(MDCK) cells with 20 mg · L-1 EGCG could inhibit influenza A replication in a time-dependent manner,probably by interrupting postbinding step.EGCG treatment could mitigate oxidative stress in influenza-infected cells and reduce the apoptosis induced by influenza infection.Conclusion:EGCG inhibits oxidative stress and apoptosis induced by influenza virus infection.

Key concepts: Oxidative stress, Epigallocatechin gallate, Flow cytometry, Apoptosis, In vitro, Virus, Influenza A virus, MTT assay

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Protection in vitro of Epigallocatechin Gallate on Oxidative Stress Induced by Influenza Infection — Research Paper | ScholarLens