Improved model of rat orthotopic renal transplantation and prevention of complications

Aim:Establishing stable rat orthotopic renal transplantation model in order to investigate the biomarkers of early acute renal allograft rejection.Methods: SD and Wistar rats were used as donors and recipients,respectively.The left orthotopic transplantation was performed.Artery end-to-side continuous sutures,renal vein end-to-end anastomosis with both continuous sutures and interrupted sutures,ureter and bladder with the bladder flap anastomosis.Results: 45 kidney transplant models were completed,with success rate 86.7%.Donor renal warm ischemia time is 30±2 min.Conclusion:The establishment of the orthotopic renal transplantation model with less complications was simple and stable which had higher achievement ratio.