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The labelling of mesenchymal stem cells mediated by recombinant replication-deficient retrovirus expressing green fluorescent protein

Shi Yongyan, Jianing Wang

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Abstract

Objective To obtain stable labelling of mesenchymal stem cells(MSCs) mediated by recombinant replication-deficient retroviral vector expressing green fluorescent protein(Rt-GFP) and provide a basis for in vivo differentiation study of MSCs in future.Methods Rat MSCs were cultured and purified with whole bone marrow method.The purified MSCs were infected with Rt-GFP.The GFP positive MSCs were observed with fluorescent microscopy.Results After Rt-GFP infection,80%~90% MSCs were labelled by GFP.The fluorescent intensity of labelled MSCs was not changeable with the prolonged culture time and the increase of passage.Conclusion MSCs may be stably labelled by Rt-GFP.

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What this paper is about

Objective To obtain stable labelling of mesenchymal stem cells(MSCs) mediated by recombinant replication-deficient retroviral vector expressing green fluorescent protein(Rt-GFP) and provide a basis for in vivo differentiation study of MSCs in future.Methods Rat MSCs were cultured and purified with whole bone marrow method.The purified MSCs were infected with Rt-GFP.The GFP positive MSCs were observed with fluorescent microscopy.Results After Rt-GFP infection,80%~90% MSCs were labelled by GFP.The fluorescent intensity of labelled MSCs was not changeable with the prolonged culture time and the increase of passage.Conclusion MSCs may be stably labelled by Rt-GFP.

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Available abstract

Objective To obtain stable labelling of mesenchymal stem cells(MSCs) mediated by recombinant replication-deficient retroviral vector expressing green fluorescent protein(Rt-GFP) and provide a basis for in vivo differentiation study of MSCs in future.Methods Rat MSCs were cultured and purified with whole bone marrow method.The purified MSCs were infected with Rt-GFP.The GFP positive MSCs were observed with fluorescent microscopy.Results After Rt-GFP infection,80%~90% MSCs were labelled by GFP.The fluorescent intensity of labelled MSCs was not changeable with the prolonged culture time and the increase of passage.Conclusion MSCs may be stably labelled by Rt-GFP.

Key concepts: Green fluorescent protein, Mesenchymal stem cell, Fluorescence microscope, Retrovirus, Recombinant DNA, Biology, Fluorescence, Bone marrow

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