Isolation and identification of pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province

According to the method of LMT,pH detection and count of the somatic cells,the epidemiology of recessive mastitis,the isolation and identification of pathogenic bacteria were carried out on 1 021 subclinical bovine from 8 bovine farms and 3 large bovine factories in Eastern Hebei Province.The results showed that the incidence rates of recessive mastitis and isolation of bacteria were 60.63%(619/1021) and 88.21%(546/619),respectively.82 strains of bacteria were isolated from 546 subclinical mastitis cows and identified as 4 classes and 14 species.The main bacteria included 36 strains of Staphylococcus,33 strains of Streptococcus,8 strains of Enterobacteria and 5 strains of Microbacterium,with the proportion of 43.90%,40.24%,9.76% and 6.10%,respectively.The main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province were Staphylococcus aureus and Streptococcus agalactiae.