Cellular toxicity of isoniazid used together with rifampicin and the metabolites of isoniazid on QSG-7701 hepatocytes

OBJECTIVE To investigate the cellular toxicity of rifampicin used together with rifampicin and the metabolites of isoniazid on cultured QSG-7701 cells lines.METHODS Isoniazid,rifampicin,rifampicin and isoniazid,acetylhydrazine,hydrazine were added in cultural media of QSG-7701 cells and cultured for 48 hours.The survival rate of cells were determined by MTT method.The cultural media and cells were collected and the activity of lactate dehydrogenase were detected with chromatometry.RESULTS Compared with control group,the survival rate decreased significantly and the lactate dehydrogenase released from cell increased significantly in cells treated with isoniazid,rifampicin,acetylhydrazine,hydrazine.Hydrazine,the metabolite of isoniazid produced significant damage on hepatocytes in low concentration.CONCLUSION Rifampicin used together with rifampicin and metabolites of isoniazid produced cellular toxic effects and hydrazine may be the most toxiferous metabolite.