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2010•Anhui nongye kexueRequires access

Detection of mRNA Expression of sprn Gene in Various Tissues of Mice by Semi-quantitative RT-PCR Method

Haiying Wang

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Abstract

[Objective] To study the expression levels and mechanisms of SPRN gene in different tissues.[Method] As many as three different mice strains were used as experimental materials.The expression levels of mRNA for SPRN gene in various tissues of different mice strains were examined by semi-quantitative reverse-transcriptase polymerase chain reaction (semi-quantitative RT-PCR),and the amplification cycles were also studied.[Result] SPRN gene was expressed mainly in brain tissue,lower in lungs and stomach,and a small amount of expression seems to be present in lymph nodes and spleen.The best results were obtained with 23 cycles of amplification.[Conclusion] A semi-quantitative RT-PCR method to detect the mRNA expression of SPRN gene has been successfully established,it is simple,rapid,accurate and high precision.

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What this paper is about

[Objective] To study the expression levels and mechanisms of SPRN gene in different tissues.[Method] As many as three different mice strains were used as experimental materials.The expression levels of mRNA for SPRN gene in various tissues of different mice strains were examined by semi-quantitative reverse-transcriptase polymerase chain reaction (semi-quantitative RT-PCR),and the amplification cycles were also studied.[Result] SPRN gene was expressed mainly in brain tissue,lower in lungs and stomach,and a small amount of expression seems to be present in lymph nodes and spleen.The best results were obtained with 23 cycles of amplification.[Conclusion] A semi-quantitative RT-PCR method to detect the mRNA expression of SPRN gene has been successfully established,it is simple,rapid,accurate and high precision.

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Available abstract

[Objective] To study the expression levels and mechanisms of SPRN gene in different tissues.[Method] As many as three different mice strains were used as experimental materials.The expression levels of mRNA for SPRN gene in various tissues of different mice strains were examined by semi-quantitative reverse-transcriptase polymerase chain reaction (semi-quantitative RT-PCR),and the amplification cycles were also studied.[Result] SPRN gene was expressed mainly in brain tissue,lower in lungs and stomach,and a small amount of expression seems to be present in lymph nodes and spleen.The best results were obtained with 23 cycles of amplification.[Conclusion] A semi-quantitative RT-PCR method to detect the mRNA expression of SPRN gene has been successfully established,it is simple,rapid,accurate and high precision.

Key concepts: Real-time polymerase chain reaction, Gene expression, Messenger RNA, Reverse transcriptase, Gene, Molecular biology, Biology, Polymerase chain reaction

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Detection of mRNA Expression of sprn Gene in Various Tissues of Mice by Semi-quantitative RT-PCR Method — Research Paper | ScholarLens