Effects of creatine phosphate on myocardium injury of limb ischemia reperfusion in rats

Objective To investigate effects and mechanisms of creatine phosphate on myocardium injury induced by limb ischemia reperfusion in rats. Methods Twenty-four rats were randomly divided into 3 groups: sham-operated control(C) group,limb ischemia reperfusion(LIR) group and creatine phosphate(P) group. The level of c-TnI,CK-MB in plasma and MDA,SOD,CaM in myocardial tissue were measured in each group. The cardiac histopathology changes in experimental rats were observed. Results In LIR group,the level of c-TnI,CK-MB in plasma and MDA,CaM in myocardial tissue were significantly increase and the contents of SOD was decreased compared with C group. And under light microscope,myocardial impairment was worst compared with the other two groups. In P group,the activity of SOD was significantly increased and the contents of MDA,CaM,c-TnI,CK-MB was decreased compared with LIR group. The cardiac histopathologic changes was better than P group. Conclusion Myocardium injury can be induced by limb ischemia-reperfusion. Creatine phosphate can protect the myocardium from limb ischemia-reperfusion,and the protective effects may be related to balance of calcium and metabolism of oxygen free radicals.