CONSTRUCTION OF A cDNA LIBRARY FROM LIVER TISSUE OF THE CHINESE SOFTSHELL TURTLE PELODISCUS SINENSIS

A high quality cDNA library from the liver of Chinese softshell turtle Pelodiscus sinensis was constructed by Superscript cDNA recombinant technique,and expressed sequence tags(ESTs) were analyzed by Gene Ontology method.The results indicate that the titre of the constructed library was 2.982×106 CFU/ml,the percentage of recombination was 93%,and the average length of inserted cDNA fragment was about 1kb.4146 raw sequences of the ESTs were obtained,1957 unigenes including 404 contigs and 1553 singletons were found.264 cDNA sequences showed similarity to known breeding-relevant genes,including ubiquitin C variant 2,heat shock protein70,metallothionein,superoxidedismutase,CD9,antibacterial peptides and so on.These results built the foundation for further researches on germplasm resources evaluation,molecular genetics studies and molecular breeding of P.sinensis.