ScholarLens Back to search
2005•Journal of Instrumental AnalysisRequires access

UV-Vis Spectrophotometric Study on the Interaction between Bromocresol Green and DNA

Jia Zhuang

Open publisher page 0 citations

Abstract

The basic conditions for the interaction between bromocresol green(BCG)and DNA were studied by UV-Vis spectrophotometry.It was found that the maximum absorbance of BCG-DNA complex was atλ 444nm .The effects of interaction time and temperature were insignificant,but varied with the pH values.The optimum pH was5.23.The results also showed that the higher the ion strength,the lower the absorbance of the complex formed,and that the foreign ions and biological agents have certain interfering effects on the abˉsorbance.The linear range of the calibration curve for the BCG-DNA complex was(0.61-7.30)×10 -7 mol/L.The apparent molar absorptivity was1.29×10 5 L·cm -1 ·mol -1 with a detection limit of7.74×10 -8 mol/L.

About this research paper

What this paper is about

The basic conditions for the interaction between bromocresol green(BCG)and DNA were studied by UV-Vis spectrophotometry.It was found that the maximum absorbance of BCG-DNA complex was atλ 444nm .The effects of interaction time and temperature were insignificant,but varied with the pH values.The optimum pH was5.23.The results also showed that the higher the ion strength,the lower the absorbance of the complex formed,and that the foreign ions and biological agents have certain interfering effects on the abˉsorbance.The linear range of the calibration curve for the BCG-DNA complex was(0.61-7.30)×10 -7 mol/L.The apparent molar absorptivity was1.29×10 5 L·cm -1 ·mol -1 with a detection limit of7.74×10 -8 mol/L.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The basic conditions for the interaction between bromocresol green(BCG)and DNA were studied by UV-Vis spectrophotometry.It was found that the maximum absorbance of BCG-DNA complex was atλ 444nm .The effects of interaction time and temperature were insignificant,but varied with the pH values.The optimum pH was5.23.The results also showed that the higher the ion strength,the lower the absorbance of the complex formed,and that the foreign ions and biological agents have certain interfering effects on the abˉsorbance.The linear range of the calibration curve for the BCG-DNA complex was(0.61-7.30)×10 -7 mol/L.The apparent molar absorptivity was1.29×10 5 L·cm -1 ·mol -1 with a detection limit of7.74×10 -8 mol/L.

Key concepts: Bromocresol green, Chemistry, Absorbance, Molar absorptivity, Spectrophotometry, Bromocresol purple, Calibration curve, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
UV-Vis Spectrophotometric Study on the Interaction between Bromocresol Green and DNA — Research Paper | ScholarLens