2009•Chinese Journal of Laboratory DiagnosisRequires access
Construction of eukaryotic expression vector pcDNA3.1-LIF and stable expression of the vector in the bone marrow derived mesenchymal stem cells
Guang Y. Yang
Open publisher page 0 citations
Abstract
Objective To contruct eukaryotic expression vector pcDNA3.1-LIF and express stablely LIF in the bone marrow derived mesenchymal stem cells.Methods LIF gene was cloned from pregnant uterine decidua tissues by using RT-PCR method and was inserted into pcDNA3.1 to construct eukaryotic expression vector consisting of LIF gene.then transfected by lipofectamine into BMSCs.LIF expression was analyzed using Western blot and RT-PCR.Results The recombinant vector pcDNA3.1-LIF was identified by restriction enzyme analysis and PCR amplification.LIF was successfully expressed in BMSCs.Conclusion The eukaryotic expression vector pcDNA3.1-LIF,which can be expressed stablely in BMSCs,has been successfully constructed.