2007•Anhui nongye kexueRequires access
Application of Response Surface Methodolgy in Optimal Bacillus subtilis Culture
Huang Yu
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Abstract
Based on the results of a Plackett-Burman design,the most important factors affecting bacteria growth of Bacillus Subtilis were further investigated with the response surface methodology.The results showed that beef extract 7.3 g/L,peptone 7.1 g/L,temperature 33.5 ℃,pH 6.1,bacteria growth mass reached the maximum.Contour plots of two-factor interaction were studied and some conclusions were obtained as follows: beef extract 5.7～8.0 g/L,peptone 5.2～8.0 g/L,temperature 33～35 ℃,pH 5.4～6.8 were best for more biomass growth.