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Study on the Factors Affecting the Agrobacterium Mediated Transformation of Brassica oleracea var.botrytis

Duan Wei

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Abstract

Taking Brassica oleracea var.botrytis variety 'Lvxiu' as material,cotyledons with petiole as explants,Agrobacterium tumefaciens strain LBA4404 were used to transformation system,and the factors affecting the Agrobacterium mediated transformation of Brassica oleracea var.botrytis were studied.The results showed that the concentration of acetosyringone,OD600 value,infection time had certain effect on transient expression.Among which OD600 value had the most influence,followed by the concentration of acetosyringone and infection time.The optimum condition was 100 μmol/L acetosyringone,OD600 value 0.3,infection time 5 min.The resistant plants demonstrated by PCR,Southern hybrization analysis and GUS staining showed that the target gene had been integrated into the broccoli genome and had been well expressed.

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What this paper is about

Taking Brassica oleracea var.botrytis variety 'Lvxiu' as material,cotyledons with petiole as explants,Agrobacterium tumefaciens strain LBA4404 were used to transformation system,and the factors affecting the Agrobacterium mediated transformation of Brassica oleracea var.botrytis were studied.The results showed that the concentration of acetosyringone,OD600 value,infection time had certain effect on transient expression.Among which OD600 value had the most influence,followed by the concentration of acetosyringone and infection time.The optimum condition was 100 μmol/L acetosyringone,OD600 value 0.3,infection time 5 min.The resistant plants demonstrated by PCR,Southern hybrization analysis and GUS staining showed that the target gene had been integrated into the broccoli genome and had been well expressed.

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Available abstract

Taking Brassica oleracea var.botrytis variety 'Lvxiu' as material,cotyledons with petiole as explants,Agrobacterium tumefaciens strain LBA4404 were used to transformation system,and the factors affecting the Agrobacterium mediated transformation of Brassica oleracea var.botrytis were studied.The results showed that the concentration of acetosyringone,OD600 value,infection time had certain effect on transient expression.Among which OD600 value had the most influence,followed by the concentration of acetosyringone and infection time.The optimum condition was 100 μmol/L acetosyringone,OD600 value 0.3,infection time 5 min.The resistant plants demonstrated by PCR,Southern hybrization analysis and GUS staining showed that the target gene had been integrated into the broccoli genome and had been well expressed.

Key concepts: Acetosyringone, Brassica oleracea, Botrytis, Biology, Agrobacterium, Agrobacterium tumefaciens, Petiole (insect anatomy), Transformation (genetics)

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Study on the Factors Affecting the Agrobacterium Mediated Transformation of Brassica oleracea var.botrytis — Research Paper | ScholarLens