Influences of TNF-α and IL-1β on peritoneal mesothelial cell adhesion molecule mRNA expression in gastric cancer

Objective To investigate the influences of TNF-α and IL-1β on peritoneal mesothelial cell adhesion molecule mRNA expression in gastric cancer. Methods The cultured vitro peritoneal mesothelial cells were as the experimental subjects,which were divided into the control group and the treatment group. The treatment group was treated with different concentrations of TNF-α and IL-1β treated cancer cells and then the cells were cultured,and the control group was treated with PBS. The cell adhesion rate was tested by flowing cytometry,and CD44,ICAM-1,VCAM-1 mRNA and gastric carcinoma cell lines ligands ICAM-1 expression of the peritoneal mesothelial cells were tested by Real-time detection. Results With the TNF-α and IL-1β concentration increasing,human peritoneal mesothelial cell adhesion molecule ICAM-1, VCAM-1 and CD44 mRNA expression levels were increased gradually,and there were statistical differences of various inflammatory factors between the treatment group and the control group( P 0. 05); the adhesion molecule LFA-1,CD44 and VLA-4 mRNA showed positive expression in human gastric cancer cell line AGS,SGC-7901,MKN-45. Conclusion Inflammatory cytokines TNF-α and IL-1β by increasing human peritoneal mesothelial cell adhesion molecule ICAM-1, VCAM-1 expression levels and CD44 mRNA can promote interaction of adhesion between gastric cancer cells and peritoneal mesothelial cells,they play positive roles in peritoneal methastasis of gastric cancer cells.