Protective Effect of Casticin on Apoptosis of HUVEC Cell Induced by Oxidative Stress

Aim To investigate the protective effective of casticin( CAS) against human umbilical vein endothelial cells( HUVEC) apoptosis induced by H2O2oxidative stress and its possible molecular mechanism. Methods HUVEC cells were cultured in vitro. The cells which would be induced by H2O2were incubated in advance for 30 minutes with ONY and various concentration of CAS. Cells viability was measured by MTT assay,and its apoptosis-inducing effect were determined by AO / EB and FCM,meanwhile western blot assay was used to measure proteins related to apoptosis. Results CAS could increase the viability of HUVEC cells induced by H2O2in a dose and time-dependent manner compared with cells solo exposed to H2O2( P 0. 05). The number of apoptotic cells and the apoptotic rate of HUVEC cells treated with various concentration CAS and H2O2significantly decreased in a dose and time-dependent manner compared with H2O2group( P 0. 05),meanwhile the expression of Cytochrome-C,Caspase-9 and Caspase-3 protein were down-regulated( P 0. 05),and protein level of Bcl-2 was activated( P 0. 05),while the expression of Bax protein showed no change in the same treatment( P 0. 05),and the ratio of Bcl-2 / Bax expression level increased( P 0. 05). Conclusion CAS could prevent H2O2-induced HUVEC cells apoptosis which might be correlated with mitochondria-controlled apoptotic pathway.