Analysis of Genetic Diversity in Lycoris radiata Using ISSR Marker

The genetic diversity of 14 Lycoris radiata populations was analyzed by ISSR markers.The results of POPGEN32 analysis showed that at species level,the percentage of polymorphics was 92.31%,Shannon's index(H) was 0.459 7,and Nei's gene diversity(I) was 0.302 5,indicating a high level of genetic diversity.But at population level,they were 49.65%,0.262 0 and 0.176 3,respectively,suggesting a low level of genetic diversity.And the gene differentiation coefficient(Gst) and gene flow(Nm) among the populations were 0.503 5 and 0.698 3,respectively.The analysis of molecular variance(AMOVA) demonstrated that there was a relatively high level genetic variation(46.12%) among the populations,and there was relatively low genetic variation(53.88%) within the populations.The high genetic differentiation among populations and the low genetic diversity within populations could be attributed to the habitat fragmentation and the limited gene flow among populations.