THE PROTECTION AND MECHANISM OF GINSENOSIDE Rg2 REPROCESSING AGAIST APOPTOSIS IN ISCHEMIA AND REPERFUSION INJURY OF MYOCARDIUM OF RAT

Objective Discuss the function and mechanism of Rg2 on myocardial ischemia-reperfusion injury in rats.Methods Choose 24 Wistar rats,divided it into normal control group(Group A),model group(Group B),Rg2 50 mg / kg group(Group C) and 100 mg / kg group(Group D),each group have six rats.Group A without any treatment,For Group B ~ D,after ligation descending branch of coronary artery 30 minutes,do reperfusion treatment on rats for 24 h so that preparing myocardial ischemia-reperfusion injury model.In next 7 days,administered some physiological saline to the rats of Group B and Rg2 50,100 mg / kg to the rats of Group C,D respectively.Then Calculate myocardial infarct size,determine superoxide dismutase(SOD) activity and malondialdehyde(MDA) in myocardial tissue,and determine myocardial endothelial-nitric oxide synthase NOS(eNOS) level by RT-PCR method.Results Compared with Group A,the infarct size,SOD activity,MDA and eNOS level of Group B ~ D have statistically significant differences(p 0.05).In group C and D,The myocardial infarct size is smaller,SOD activity and MDA、eNOS level is higher than group B(p 0.05 or p 0.01).Conclusion Ginsenoside Rg2 has significant protective effect on experimental myocardial ischemia-reperfusion injury,maybe because antioxidant activity increase,free radical oxidative damage reduced or eNOS increase in the organism.