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Optimization of extraction technique of protein from lotus seed using response surface methodology

WU Wei-yun

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Abstract

The extraction technique of proteins from lotus seed was optimized using Plackett-Burman design,path of steepest ascent and response surface methodology.The results showed that the optimum extraction condition was 3.27% SDS concentration,0.2% DTT concentration,pH=12.0,12∶1(V/W)of solvent/material ratio,and ultrasonic assistant treatment 21.5 min,extracting in 30℃ for 90 min.The extraction yield of protein from lotus seed could reach 92.27% under this optimum extraction condition.

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The extraction technique of proteins from lotus seed was optimized using Plackett-Burman design,path of steepest ascent and response surface methodology.The results showed that the optimum extraction condition was 3.27% SDS concentration,0.2% DTT concentration,pH=12.0,12∶1(V/W)of solvent/material ratio,and ultrasonic assistant treatment 21.5 min,extracting in 30℃ for 90 min.The extraction yield of protein from lotus seed could reach 92.27% under this optimum extraction condition.

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Available abstract

The extraction technique of proteins from lotus seed was optimized using Plackett-Burman design,path of steepest ascent and response surface methodology.The results showed that the optimum extraction condition was 3.27% SDS concentration,0.2% DTT concentration,pH=12.0,12∶1(V/W)of solvent/material ratio,and ultrasonic assistant treatment 21.5 min,extracting in 30℃ for 90 min.The extraction yield of protein from lotus seed could reach 92.27% under this optimum extraction condition.

Key concepts: Response surface methodology, Extraction (chemistry), Chromatography, Yield (engineering), Lotus, Solvent, Lotus effect, Protein purification

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