Expression and its significance of Caspase-3、Bcl-2 and PCNA in the various pathological type of IgA nephropathy

Objective To investigate the expression and significance of Caspase-3、Bcl-2 and PCNA in the various pathological type of IgA nephropathy(IgAN),and discuss the relationship between clinical index and Caspase-3、Bcl-2 and PCNA.Methods The expression of PCNA、Caspase-3 and Bcl-2 in 54 cases of renal setions was examined by immunohistochemistry.Collected the results of clinical indexes:serum creatinine(Scr)、urea nitrogen(BUN)、24 hour urine protein quant.Results(1)In IgA renal tissue the expression of PCNA from low to high were levelⅠ(0.09±0.03)、levelⅡ(0.12±0.02)、levelⅤ(0.13±0.01)、level Ⅲ(0.28±0.08)、level Ⅳ(0.54±0.03),between grade Ⅰ and grade Ⅱ、Ⅲ、Ⅳ、Ⅴ,the expression of PCNA were both significantly different(P 0.01).The expression of Caspase-3 and Bcl-2 were both gradely increased followed the level of IgAN.The expression of Caspase-3 in turn were 0.08±0.01、0.11±0.02、0.13±0.08、0.20±0.01、0.35±0.01,between level Ⅰand other levels which were both statistically significant(P 0.01).The expression of Bcl-2 in turn were 0.09±0.02、0.11±0.01、0.15±0.01、0.29±0.01、0.32±0.01,between grade Ⅰ、Ⅱand grade Ⅲ、Ⅳ、Ⅴ which were both statistically significant(P 0.01).(2)From grade Ⅰ to Ⅴ,Scr、 BUN、 24 hour urine protein quant were gradually increased,Scr levels were 65.08±14.11、81.53±21.71、133.86±69.36、147.56±52.69、280.03±137.81,its level between gradeⅤand other groups were significantly different(P 0.01);BUN levels were 4.77±1.39、6.16 ±2.48、6.97±3.60、9.44±2.80、13.30±6.32,its level between grade Ⅴ and grade Ⅰ、Ⅱ、Ⅲ were significantly different(P 0.01);24 hour urine protein quant levels were 0.84±0.33、2.50±1.08、3.35±0.82、3.54±0.77、4.66±1.42,its level between grade Ⅰ and other levels and between grade Ⅴ and gradeⅡ、Ⅲ were all significantly different(P 0.01).(3)The expression of PCNA、Caspase-3 and Bcl-2 were positively correlated with the level of Scr、BUN and 24 hour urine protein quant(P 0.01).The expression of PCNA was positively correlated with Caspase-3.Conclusion The expressions of PCNA、Caspase-3 and Bcl-2 are gradually increased with ⅠtoⅤIgA nephropathy.The progression of IgAN and the damage of renal function may be connected with the imbalance between proliferation and apoptosis.