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2010•Journal of Qiqihar UniversityRequires access

Protocols of application of PCR-DGGE in studies of fungal community

Zhai Zu-huan

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Abstract

Introducing detailed procedures for the application denaturing gradient gel electrophoresis(DGGE) in the study of fungal community.The main procedure for DGGE includes: Extracting DNA directly from samples,PCR in the region of 18SrDNA or IST sequence using a primer with GC clamp in 5′,analyzing the PCR products in denaturing gradient polyacrylamide gel with a chemical denaturing gradient which could separate the different DNA fragments and then extracting bands from the gel.

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What this paper is about

Introducing detailed procedures for the application denaturing gradient gel electrophoresis(DGGE) in the study of fungal community.The main procedure for DGGE includes: Extracting DNA directly from samples,PCR in the region of 18SrDNA or IST sequence using a primer with GC clamp in 5′,analyzing the PCR products in denaturing gradient polyacrylamide gel with a chemical denaturing gradient which could separate the different DNA fragments and then extracting bands from the gel.

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Available abstract

Introducing detailed procedures for the application denaturing gradient gel electrophoresis(DGGE) in the study of fungal community.The main procedure for DGGE includes: Extracting DNA directly from samples,PCR in the region of 18SrDNA or IST sequence using a primer with GC clamp in 5′,analyzing the PCR products in denaturing gradient polyacrylamide gel with a chemical denaturing gradient which could separate the different DNA fragments and then extracting bands from the gel.

Key concepts: Temperature gradient gel electrophoresis, Primer (cosmetics), Chromatography, Gel electrophoresis, Polyacrylamide gel electrophoresis, DNA, Polymerase chain reaction, Biology

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