Screening of toluene degrading strains and its application in bioaugmentation toluene degradation

Toluene-degradating strains were isolated from petroleum-contaminated soil of Dagang oilfield.Eight bacterium were screened with the method of enrichment and dilution,which were identified as Bacillus aquimaris,Enterobacter,Pseudomonas putida,Microbacterium,Arthrobacter,Microbacterium,Staphylococcu,Planococcus rifietoensis by 16S rDNA identification,and named as TD1-TD8 respectively.Among the eight bacteria,TD3 presented best performance for toluene degradation,and the max degradation rate was 96.3%.The optimum growth conditions were pH=7 and 30 ℃ of temperature.TD3 were added in the activated sludge of the Hangu chemical wastewater treatment plant for bioagmentation degradation of toluene,the biodegradation rate of toluene was increased from 86.0% to 94.5%.